No, on the Denon after you run audyssey all of the remaining settings remain in place even if you go adjust the distance on one speaker or many. When audyssey runs you can set up two different presets but you can always adjust the readings as you see fit. I ran audyssey only using 3 positions for preset 1 and all positions for preset 2. I am experimenting between the two presets to see if audyssey is more or less accurate using the different numbers of positions. Now that I have my M5s hooked up and the listening position adjusted I need to re-run my audyssey settings but I will still run it twice using the different number of positions.
LFR1100 VP180HP EP600 M3 In Ceiling x 4 M5OW M2 - Atmos rears ADA1500.5 M3 Outdoor Speakers ADA 1000.8